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Temperature at the rear cyl is higher, main reason being that it is hidden by the front cyl. And maybe, don't quote me on this, but the rear cyl fires a split second later than the front.
You can do this with most programming tuners (TTS, SEPST, etc). Its just that its usually done with one AFR table and then the difference in fueling is adjusted in the cylinder-specific VE tables.
This is one of the nice differences between carb and EFI, of course. With a carb you throw the fuel in the manifold and it, hopefully, gets split evenly between the cylinders. With sequential port fuel injection, each cylinder has its own injectors near the intake valves.
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